Journal: The Journal of Experimental Biology

Article Title: Circadian coupling of mitochondria in a deep-diving mammal

doi: 10.1242/jeb.246990

Figure Lengend Snippet: Clock genes expression profile in synchronized hooded seals fibroblasts and tissues. (A) mRNA expression of core clock genes arntl (blue line) and per2 (black line) in hooded seal fibroblasts synchronized by temperature cycle (°C), as represented in the upper panel. Arntl and per2 mRNAs show a 24-h period and are in antiphase. P -values and phases were calculated through JTK cycle analysis ( n =4). (B) mRNA expression of core clock genes arntl (blue line) and per2 (black line) in hooded seal fibroblasts synchronized by temperature cycling and then left at constant temperature (upper panel). Arntl and per2 mRNAs maintain a 24-h period and their antiphasic relationship also in constant conditions. P -values and phases were calculated through JTK cycle analysis ( n =4). (C) Photon multiplier tube recordings of hooded seal skin fibroblasts transfected with arntl :luciferase (blue line) and per2 :luciferase (black line). Cells were synchronized with dexamethasone. (D) Arntl mRNA expression in hooded seal kidney and liver tissue, sampled in mid-light phase (ZT6) and mid-dark phase (ZT18). * P <0.05 (one-way ANOVA; n =3 except liver ZT18, where n =2). Data are expressed as means±s.e.m.

Article Snippet: The latter consisted of the pLV6 backbone and a mouse per2 promoter with adjacent luciferase sequence contained in the pGL3 basic E2 vector (Addgene plasmid 48747).

Techniques: Expressing, Transfection, Luciferase

Journal: Cell Death and Differentiation

Article Title: PAK5 promotes the migration and invasion of cervical cancer cells by phosphorylating SATB1

doi: 10.1038/s41418-018-0178-4

Figure Lengend Snippet: PAK5-mediated phosphorylation promotes EMT in cervical cancer cells. a, b Effects of SATB1 and SATB1 Ser47A on the migration and invasion ability in HeLa and SiHa cells. Scale bar, 100μm for a and b. c HeLa and SiHa cells were transfected with pGL3-E, together with the indicated expression plasmids for Dual luciferase reporter assays. d Effects of SATB1 knockdown on EMT markers were analyzed by western blotting in HeLa cells overexpressing PAK5. e Effects of SATB1 overexpression on EMT markers in HeLa cells silencing PAK5. Data are showed as mean ± SD for three independent experiments. **, P < 0.01; ***, P < 0.001. pGL3-E pGL3-E-cadherin promoter, siCtrl siRNA control, siPAK5 siRNA PAK5, ns not significant, GAPDH glyceraldehyde-3-phosphate dehydrogenase

Article Snippet: Briefly, E-cadherin promoter was subcloned into pGL3-Basic luciferase expression vector (Genepharma).

Techniques: Migration, Transfection, Expressing, Luciferase, Western Blot, Over Expression